LIBS in Microbiology


Identification of microbiological objects such as bacteria, fungi, and pollens has become a critical task for medical, biological, and environmental surveillance. Several methods have been proposed for the identification of bacterial strain, based on molecular techniques. Unfortunately, as well know these techniques present difficulties as use of consumables, and expensive and complex instrumentation. It is also important to remark the importance of sample preparation due to the time consumption and the cost. Them is understandably the increasing need for fast and precise techniques for microbiological sample identification.


The Potential of Compact Libs System with Multi-Pulse Nd: YAG Laser for Bacteria Identification

Between the analytical techniques for microanalysis, Laser Induced-Breakdown Spectroscopy (LIBS) has considered one of the most promising for field applications. This is an atomic emission spectroscopy technique for material characterization that is based on a laser beam that generates plasma upon irradiating a sample. Unlike other atomic emission spectroscopy methods, the source of excitation is a laser pulse, providing various advantages over other techniques. Among these advantages, the system can detect almost any element, does not require prior sample preparation, and enables fast detection; in addition, because of its portability, it is possible to perform an in-situ or nearby analysis for inaccessible samples [4]. Thanks to all these advantages, this technique has gained many applications during the last years.




The spectrum of two species of bacteria obtained with a pulse shot.

(a) Escherichia coli

(b) Staphylococcus aureus