Rapid identification of Huanlongbing-infected citrus plants using LIBS

Updated: Mar 15

Huanglongbing (HLB) is the most destructive disease of citrus worldwide. The disease is caused by the proto-bacteria Candidatus Liberibacter asiaticus and transmitted by the Asian citrus psyllid Diaphorina citri. HLB symptoms are slow to appear, while the tree continues to be a source of inoculum. Monitoring tree health and rapid detection of HLB is critical for sustainable citrus production. Nowadays, scientists are working on developing new techniques for pre-symptomatic detection of HLB, as there is no available method for real-time assessment of tree health. In this study, we demonstrate the rapid and efficient discrimination between healthy and HLB-affected of citrus by laser-induced breakdown spectroscopy (LIBS) combined with chemometric analysis. Healthy and HLB-affected trees were differentiated with a high degree of precision. The novelty of this method lies in the fingerprinting of healthy and diseased plants based on their organic and inorganic constituents, and the use of a multi-pulse laser coupled with a microscope to take spectra of the plant phloem.

The LIBS, -Laser Induced Breakdown Spectroscopyis a Compositional microanalysis technique, with strong development in recent years. It is based on the use of a laser that vaporizes a small portion of material (leaving a crater of microns in diameter) to form a plasma which is studied by an optical spectrometer. Thanks to the high power of the laser pulse used, highly localized and focused, plasma may be generated from virtually any material, thus revealing the constituents of the sample.
Complete LIBS Measurement System for UV, Visible, and IR Wavelengths


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